Oracle VirtualBox is a cross-platform virtualization application. It installs on your existing Intel or AMD-based computers, whether they are running Windows, Mac, Linux or Solaris operating systems. VirtualBox can create and run a 'guest' operating system (virtual machine) in a window of the host operating system. The virtual machine provides a self-contained environment in which to experiment with new software without risking damaging changes to the host operating system.

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1. Regardless how incredible is the idea to run any operational system on your PC, it is still true. By using the virtual emulator, you can install literally any system on your Windows-based computer: Linux, Unix, FreeBSD, Android, Mac OS X, even not mentioning all the systems from Windows generation: from ancient Win 95 and 98 builds to the latest Win 8.1 and Win 10 releases (both 32- or 64-bit).
2. Install WiX 311 Link. Install binaries version to C: Programs WiX. Install cygwin Link. Install x64 to C: Programs cygwin64. Add the packages make and yasm. Download and extract Qt 5.6.3 source Link. Extract to C: Programs Qt. Rename folder extracted to 5.6.3-src. Create folder build-x32 and build-x64 next to it.
3. Download Windows 10 Disc Image (ISO File) Before updating. Check these things on the PC where you want to install Windows 10: 64-bit or 32-bit processor (CPU).
4. VirtualBox is a multi platform and open-source virtualization tool for Windows, Linux, Mac OS X, and other operating systems, that lets you create virtual disc units in which you can install a guest operating.

Download VirtualBox for Windows 10 32 bit/ 64 bit and how to install Windows 10 on a VirtualBox Virtual Machine. VirtualBox is a virtual application that allows you to install other or additional Operating Systems and run concurrently on the parent operating system. 4 Type a name (ex: Windows 10 build 10166) you want for the Windows 10 virtual.

We will give an instruction with step-by-step screenshots to show how to install VirtualBox with an example of installing Ubuntu OS as a guest machine. We also explain how to share files between the host and guest operating systems.

Downloads

Note the instruction here is based on the latest version of the VirtualBox. If you have already installed an earlier version of VirtualBox, your experience may be different. It is recommended to use the latest version of VirtualBox to avoid issues. The VirtualBox forum is a good place to find a solution if you see any problems.

Go to VirtualBox website here to download the binary for your current operating system. Since our host machine is running on Windows, I'll choose 'x86/amd64' from Windows hosts. When download is finished, run the executable file. Continue with the installation of VirtualBox with the defaults. This will open VirtualBox at the end of the installation.

Create Virtual Machine

Click 'New' button to open a dialog.

Type a name for the new virtual machine. Since I am planning to install Ubuntu 14.04, I'll enter 'ubuntu1404'. Note that VirtualBox automatically changes 'Type' to Linux and 'Version' to 'Ubuntu (64 bit)'. These two options are exactly what we need.

The memory size depends on your host machine memory size. In my case, I have 12GB physical RAM. I like to allocate as much as possible for Ubuntu but leave some for my Windows host machine. I pick 8192 MB for my Ubuntu. Note that VirtualBox will create a swap partition with the same amount space as base memeory you have entered here. So later when you are selecting the size of the virtual hard drive, make sure it is large enough since the hard drive will be splitted into root (/)and swap partitions. The root partition contains by default all your system files, program settings and documents.

Accept the default 'Create a virtual hard drive now' and click 'Create' button.

Continue to accept the default 'VDI' drive file type and click 'Next' button.

Change the storage type from the default 'Dynamically allocated' to 'Fixed size' to increase performance.

For the virtual hard drive space, the default value is 8GB which is too little for RNA-Seq analysis. I'll pick 100GB since I have plenty of space in my hard disk. You want to choose a good size for your RNA-Seq analysis. If you realize the drive space is not large enough, you'll need to go over these steps again to create another virtual machine.

Click 'Create' button and VirtualBox will generate Ubuntu virtual machine.

Now the virtual machine is created. We are ready to install Ubuntu in this virtual machine. Select your new virtual machine and click 'Settings' button. Click on 'Storage' category and then 'Empty' under Controller:IDE. Click 'CD/DVD' icon on right hand side and select the ubuntu ISO file to mount.

Note that if you have not downloaded 64-bit Ubuntu ISO file, you can check out this page for more information. When downloading Ubuntu ISO file, make sure to selecte 64-bit version. Also make sure the VT-x/Virtualization Technology has been enabled in your computer's BIOS/Basic Input Output System.

Since Tophat program can take an advantage of multiple processors/threads, it is a good idea to specify a large number of processors in virtual machine (default value is 1). You can change this number by clicking on 'System' category. In this case, I change the number of CPUs to 4 since 4 is the largest value shown on the green bar in my case. Now you can click 'OK' button to continue.

VirtualBox may pop up a message about 'Auto capture keyboard' option. Read the message there and check 'Do not show this message again' option before clicking OK.

Install Ubuntu

Back to Oracle VM VirtualBox Manager, click on the new Ubuntu virtual machine and hit 'Start' button. Now you shall see a 'Welcome' screen. Click 'Install Ubuntu' button. Note that the installation process may differ a little bit from version to version. The screenshots here are based on Ubuntu 14.04.1.

Click 'Continue' button.

Make sure 'Erase disk and install Ubuntu' option is selected and click 'Install Now' button.

Ubuntu will ask you a few questions. If the default is good, click 'Continue' button.

In 'Who are you?' dialog, enter your preferred name, username and password. Note that this user will have root/sudo privilege. Click 'Continue' button.

The installation will continue until it is finished.

After installation is complete, click 'Restart Now' button. When you see a screen with a black background saying 'Please remove installation media and close the tray (if any) then press ENTER:', just follow it.

Enter the password you have chosen and press 'Enter'.

The Ubuntu Desktop OS is ready. You may find the desktop screen is too small. Don't worry. You can solve this easily with 'VirtualBox Guest Additions'.

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Guest Additions

After the Ubuntu guest machine is installed, you will find the Ubuntu OS screen size is not extendable and you are not able to share folders between the host machine and Ubuntu.

Guest Additions software provide additional capability to a guest virtual machine, including mouse pointer integration, better video support, share folders, share clipboard, et al.

Before we proceed to install Guest Additions, make sure the current user has sudo/root privilege. If the current user does not have sudo/root privilege or it is not sure, run the following terminal command from an account (such as the user created when Ubuntu was first created; see the screenshot of 'Who are you?' above) with root privileges to enable the root privilege for the current user where USERNAME should be replaced by the current user's name.

To install Guest Additions, click Devices > Insert Guest Additions CD images…

Click 'Run' button (next to 'Cancel' button) to start the installation. Note that the version of VBOXADDITIONS should be matched with the one of VirtualBox you have installed.

Installing Guest Additions requires root privilege. Enter user's password (assume the current user has sudo privilege).

When the installation is finished, press Return key to close the terminal window.

Now prepare to restart the system to enable the change. Click 'Shutdown' on the menu and then the 'Restart' button.

After rebooting Ubuntu, you will see the desktop resolution is much better.

Share Folders

Before you can enable shared folders on the host machine, make sure you have installed VirtualBox Guest Additions software on the guest machine.

When Ubuntu guest machine is on, click on Devices > Shared folders settings…

Select Machine Folders and hit the add button (plus sign).

Browse to the folder you wish to access from the guest machine. Check Auto-mount and Make Permanent options and click OK button.

Click OK button once more.

Now type 'terminal' in Dash to open a Terminal.

Type the following line in the Terminal to add a user to 'vboxsf' group. This step is necessary in order to use the VB's 'Shared Folder' feature.Replace 'brb' with your account name in Ubuntu.When you are done, restart the Ubuntu guest machine and go to /media/ directory. From Nautilus (file manager in Ubuntu), click Computer > File System > Media folder and inside it you will see a folder beginning with sf_ (the folder name is sf_Downloads in our example). Now you can transfer files between the Ubuntu guest machine and the Windows host machine in Nautilus.

Although Shared Folder system in VirtualBox is a nice feature, using VirtualBox shared folder directly for fastq data, annotation or output directorycan significantly reduce the performance compared to a native (Ubuntu) system or VirtualBox native system.

BRB-SeqTools

After Ubuntu has been successfully installed on VirtualBox, users can be go back to download and use BRB-SeqTools program.

This tutorial will show you step by step what to do when VirtualBox is only showing 32-bit and no 64-bit versions of operating systems as options while creating a new virtual machine.

Note: This may be obvious for most of you, but your host operating system should be 64-bit to run 64-bit guest operating systems in VirtualBox.

If VirtualBox has no 64-bit option on Windows 10, then you can try the solutions on this page to fix the problem.

Methods:

My experience with this problem

I experienced the same problem while trying to install a 64-bit version of Linux Mint inside VirtualBox.

I opened VirtualBox, clicked on NEW, entered a name for the virtual machine, choose the type of operating system, but when I wanted to choose a version, I only saw 32-bit versions and NO 64-bit versions as an option.

But:

Fortunately, I found a simple solution online and on this page, I will share that solution with you.

Note! Your host operating system (OS installed on your PC) must be 64-bit.

I was using a 64-bit version of Microsoft Windows 10 Pro while creating this tutorial.

Make sure that “Intel Virtualization Technology” and “VT-d” are enabled

Enter BIOS settings and check if “Intel Virtualization Technology” and “VT-d” are enabled.

The following steps may be slightly different on your computer.

1. Start (or restart) your PC.

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2. Press the right keyboard key to enter the BIOS setup. This must be done before Windows starts, so it's best to press the key a few times directly after you start your PC.

The exact key or key combination depends on your computer manufacturer and model.

Common keys to enter the BIOS are F1, F2, F10, DEL, and ESC.

3. Look for the following settings Intel Virtualization Technology and VT-d.

4. Make sure that they're both enabled.

5. If you made changes, then you must save the settings.

Windows 10 (host) must be a 64-bit operating system

To check if your Windows 10 is a 64-bit operating system, press the [Windows] + [pause] keyboard keys.

At System type (located under System), you should see something like 64-bit Operating System, x64-based processor.

Hyper-V must be disabled

Hyper-V is Microsoft's hardware virtualization product.

It lets you create and run virtual machines (like VirtualBox).

But:

Many third-party virtualization applications (including VirtualBox) do not work together with Hyper-V.

That's why you must make sure that Hyper-V is disabled.

To check if Hyper-V is disabled, follow the following steps:

1. Right-click on the Windows start menu button.

2. Click on Run.

3. Enter optionalfeatures.exe in the Run window.

4. Click on OK or press Enter.

The Windows Features window will appear.

5. Check if the Hyper-V option is unchecked.

6. If the Hyper-V option unchecked (like in the screenshot above), then you can close the Windows Features window.

But:

If the option is checked, then you will need to uncheck it and then click on OK.

If you want to be 100% sure that Hyper-v is disabled then follow these steps:

1. Right-click on the Windows start menu button.

2. Click on Command Prompt (Admin).

3. Enter the following command:

4. Press Enter.

5. After the command is finished, you will need to power down and reboot your PC.

Solution 1: Repair VirtualBox installation

1. Right-click on the installer file (the one you used to install VirtualBox) and choose Run as administrator.

If you don't have the installer file anymore, then you will need to download it from the VirtualBox website (www.virtualbox.org). The installer file must be the same version as the version of VirtualBox that's installed on your computer.

2. When the Windows User Account Control window appears, you click Yes.

Note: You might need to enter an administrator password in the User Account Control window and then click Yes.

A setup window will appear.

3. Click on Next.

4. Click on the Repair icon.

5. Click on Repair located at the bottom right.

VirtualBox will now start the repairing process. This will only take a few seconds.

6. After the repair process is finished, you click on Finish.

VirtualBox may ask you to restart your PC.

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Hopefully, this simple solution will solve your problem.

Solution 2: Disable “Memory integrity” in Windows 10

1. Open Windows Security.

2. Click on Device security.

3. Under Core isolation, you click on Core isolation details.

4. Make sure that Memory integrity is turned off.

If Memory integrity was enabled and you just disabled it, then you may need to restart your PC.

Hopefully, this simple solution will solve your problem.

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